PoplarV1, Main, Exploration, bibRecord, 001198

Silencing CAFFEOYL SHIKIMATE ESTERASE Affects Lignification and Improves Saccharification in Poplar.

Identifieur interne : 001198 ( Main/Exploration ); précédent : 001197; suivant : 001199

Silencing CAFFEOYL SHIKIMATE ESTERASE Affects Lignification and Improves Saccharification in Poplar.

Auteurs : Marina De Lyra Soriano Saleme [Belgique] ; Igor Cesarino [Belgique, Brésil] ; Lívia Vargas [Belgique] ; Hoon Kim [États-Unis] ; Ruben Vanholme [Belgique] ; Geert Goeminne [Belgique] ; Rebecca Van Acker [Belgique] ; Fernando Campos De Assis Fonseca [Belgique] ; Andreas Pallidis [Belgique] ; Wannes Voorend [Belgique] ; José Nicomedes Junior [Belgique, Brésil] ; Dharshana Padmakshan [États-Unis] ; Jan Van Doorsselaere [Belgique] ; John Ralph [États-Unis] ; Wout Boerjan [Belgique]

Source :

Plant physiology [ 1532-2548 ] ; 2017.

RBID : pubmed:28878037

Descripteurs français

KwdFr :
- Acide shikimique (métabolisme), Biomasse (MeSH), Cellulose (métabolisme), Développement des plantes (génétique), Esterases (métabolisme), Extinction de l'expression des gènes (MeSH), Gènes de plante (MeSH), Lignine (métabolisme), Métabolisme glucidique (génétique), Phénols (métabolisme), Populus (croissance et développement), Populus (enzymologie), Populus (génétique), Protéines végétales (métabolisme), Régulation de l'expression des gènes végétaux (MeSH), Régulation négative (génétique), Spectroscopie par résonance magnétique (MeSH), Voies et réseaux métaboliques (MeSH), Végétaux génétiquement modifiés (MeSH), Xylème (métabolisme).
MESH :
- croissance et développement : Populus.
- enzymologie : Populus.
- génétique : Développement des plantes, Métabolisme glucidique, Populus, Régulation négative.
- métabolisme : Acide shikimique, Cellulose, Esterases, Lignine, Phénols, Protéines végétales, Xylème.
- Biomasse, Extinction de l'expression des gènes, Gènes de plante, Régulation de l'expression des gènes végétaux, Spectroscopie par résonance magnétique, Voies et réseaux métaboliques, Végétaux génétiquement modifiés.

English descriptors

KwdEn :
- Biomass (MeSH), Carbohydrate Metabolism (genetics), Cellulose (metabolism), Down-Regulation (genetics), Esterases (metabolism), Gene Expression Regulation, Plant (MeSH), Gene Silencing (MeSH), Genes, Plant (MeSH), Lignin (metabolism), Magnetic Resonance Spectroscopy (MeSH), Metabolic Networks and Pathways (MeSH), Phenols (metabolism), Plant Development (genetics), Plant Proteins (metabolism), Plants, Genetically Modified (MeSH), Populus (enzymology), Populus (genetics), Populus (growth & development), Shikimic Acid (metabolism), Xylem (metabolism).
MESH :
- chemical , metabolism : Cellulose, Esterases, Lignin, Phenols, Plant Proteins, Shikimic Acid.
- enzymology : Populus.
- genetics : Carbohydrate Metabolism, Down-Regulation, Plant Development, Populus.
- growth & development : Populus.
- metabolism : Xylem.
- Biomass, Gene Expression Regulation, Plant, Gene Silencing, Genes, Plant, Magnetic Resonance Spectroscopy, Metabolic Networks and Pathways, Plants, Genetically Modified.

Abstract

Caffeoyl shikimate esterase (CSE) was recently shown to play an essential role in lignin biosynthesis in Arabidopsis (Arabidopsis thaliana) and later in Medicago truncatula However, the general function of this enzyme was recently questioned by the apparent lack of CSE activity in lignifying tissues of different plant species. Here, we show that down-regulation of CSE in hybrid poplar (Populus tremula × Populus alba) resulted in up to 25% reduced lignin deposition, increased levels of p-hydroxyphenyl units in the lignin polymer, and a relatively higher cellulose content. The transgenic trees were morphologically indistinguishable from the wild type. Ultra-high-performance liquid chromatography-mass spectrometry-based phenolic profiling revealed a reduced abundance of several oligolignols containing guaiacyl and syringyl units and their corresponding hydroxycinnamaldehyde units, in agreement with the reduced flux toward coniferyl and sinapyl alcohol. These trees accumulated the CSE substrate caffeoyl shikimate along with other compounds belonging to the metabolic classes of benzenoids and hydroxycinnamates. Furthermore, the reduced lignin amount combined with the relative increase in cellulose content in the CSE down-regulated lines resulted in up to 62% more glucose released per plant upon limited saccharification when no pretreatment was applied and by up to 86% and 91% when acid and alkaline pretreatments were used. Our results show that CSE is not only important for the lignification process in poplar but is also a promising target for the development of improved lignocellulosic biomass crops for sugar platform biorefineries.

DOI: 10.1104/pp.17.00920
PubMed: 28878037
PubMed Central: PMC5664470

Affiliations:

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Le document en format XML

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 Affects Lignification and Improves Saccharification in Poplar.</title>
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<author><name sortKey="Fonseca, Fernando Campos De Assis" sort="Fonseca, Fernando Campos De Assis" uniqKey="Fonseca F" first="Fernando Campos De Assis" last="Fonseca">Fernando Campos De Assis Fonseca</name>
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<author><name sortKey="Junior, Jose Nicomedes" sort="Junior, Jose Nicomedes" uniqKey="Junior J" first="José Nicomedes" last="Junior">José Nicomedes Junior</name>
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</affiliation>
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<author><name sortKey="Padmakshan, Dharshana" sort="Padmakshan, Dharshana" uniqKey="Padmakshan D" first="Dharshana" last="Padmakshan">Dharshana Padmakshan</name>
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<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Wisconsin</region>
</placeName>
<wicri:cityArea>Department of Energy Great Lakes Bioenergy Research Center, Wisconsin Energy Institute, University of Wisconsin, Madison</wicri:cityArea>
</affiliation>
<affiliation wicri:level="2"><nlm:affiliation>Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Wisconsin</region>
</placeName>
<wicri:cityArea>Department of Biochemistry, University of Wisconsin, Madison</wicri:cityArea>
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<author><name sortKey="Van Doorsselaere, Jan" sort="Van Doorsselaere, Jan" uniqKey="Van Doorsselaere J" first="Jan" last="Van Doorsselaere">Jan Van Doorsselaere</name>
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<author><name sortKey="Ralph, John" sort="Ralph, John" uniqKey="Ralph J" first="John" last="Ralph">John Ralph</name>
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<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Wisconsin</region>
</placeName>
<wicri:cityArea>Department of Energy Great Lakes Bioenergy Research Center, Wisconsin Energy Institute, University of Wisconsin, Madison</wicri:cityArea>
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<affiliation wicri:level="2"><nlm:affiliation>Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Wisconsin</region>
</placeName>
<wicri:cityArea>Department of Biochemistry, University of Wisconsin, Madison</wicri:cityArea>
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<author><name sortKey="Boerjan, Wout" sort="Boerjan, Wout" uniqKey="Boerjan W" first="Wout" last="Boerjan">Wout Boerjan</name>
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<wicri:regionArea>Ghent University, Department of Plant Biotechnology and Bioinformatics, 9052 Ghent</wicri:regionArea>
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<placeName><settlement type="city">Gand</settlement>
<region>Région flamande</region>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">Silencing <i>CAFFEOYL SHIKIMATE ESTERASE</i>
 Affects Lignification and Improves Saccharification in Poplar.</title>
<author><name sortKey="Saleme, Marina De Lyra Soriano" sort="Saleme, Marina De Lyra Soriano" uniqKey="Saleme M" first="Marina De Lyra Soriano" last="Saleme">Marina De Lyra Soriano Saleme</name>
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<placeName><settlement type="city">Gand</settlement>
<region>Région flamande</region>
<region type="district" nuts="2">Province de Flandre-Orientale</region>
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</affiliation>
<affiliation wicri:level="1"><nlm:affiliation>VIB Center for Plant Systems Biology, 9052 Ghent, Belgium.</nlm:affiliation>
<country xml:lang="fr">Belgique</country>
<wicri:regionArea>VIB Center for Plant Systems Biology, 9052 Ghent</wicri:regionArea>
<wicri:noRegion>9052 Ghent</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Cesarino, Igor" sort="Cesarino, Igor" uniqKey="Cesarino I" first="Igor" last="Cesarino">Igor Cesarino</name>
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<country xml:lang="fr">Belgique</country>
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<affiliation wicri:level="1"><nlm:affiliation>VIB Center for Plant Systems Biology, 9052 Ghent, Belgium.</nlm:affiliation>
<country xml:lang="fr">Belgique</country>
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</affiliation>
<affiliation wicri:level="4"><nlm:affiliation>Department of Botany, Institute of Biosciences, University of São Paulo, 05508-090 Butanta, Sao Paulo, Brazil.</nlm:affiliation>
<country xml:lang="fr">Brésil</country>
<wicri:regionArea>Department of Botany, Institute of Biosciences, University of São Paulo, 05508-090 Butanta, Sao Paulo</wicri:regionArea>
<placeName><settlement type="city">São Paulo</settlement>
<region type="state">État de São Paulo</region>
</placeName>
<orgName type="university">Université de São Paulo</orgName>
</affiliation>
</author>
<author><name sortKey="Vargas, Livia" sort="Vargas, Livia" uniqKey="Vargas L" first="Lívia" last="Vargas">Lívia Vargas</name>
<affiliation wicri:level="4"><nlm:affiliation>Ghent University, Department of Plant Biotechnology and Bioinformatics, 9052 Ghent, Belgium.</nlm:affiliation>
<country xml:lang="fr">Belgique</country>
<wicri:regionArea>Ghent University, Department of Plant Biotechnology and Bioinformatics, 9052 Ghent</wicri:regionArea>
<orgName type="university">Université de Gand</orgName>
<placeName><settlement type="city">Gand</settlement>
<region>Région flamande</region>
<region type="district" nuts="2">Province de Flandre-Orientale</region>
</placeName>
</affiliation>
<affiliation wicri:level="1"><nlm:affiliation>VIB Center for Plant Systems Biology, 9052 Ghent, Belgium.</nlm:affiliation>
<country xml:lang="fr">Belgique</country>
<wicri:regionArea>VIB Center for Plant Systems Biology, 9052 Ghent</wicri:regionArea>
<wicri:noRegion>9052 Ghent</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Kim, Hoon" sort="Kim, Hoon" uniqKey="Kim H" first="Hoon" last="Kim">Hoon Kim</name>
<affiliation wicri:level="2"><nlm:affiliation>Department of Energy Great Lakes Bioenergy Research Center, Wisconsin Energy Institute, University of Wisconsin, Madison, Wisconsin 53726.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Wisconsin</region>
</placeName>
<wicri:cityArea>Department of Energy Great Lakes Bioenergy Research Center, Wisconsin Energy Institute, University of Wisconsin, Madison</wicri:cityArea>
</affiliation>
<affiliation wicri:level="2"><nlm:affiliation>Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Wisconsin</region>
</placeName>
<wicri:cityArea>Department of Biochemistry, University of Wisconsin, Madison</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Vanholme, Ruben" sort="Vanholme, Ruben" uniqKey="Vanholme R" first="Ruben" last="Vanholme">Ruben Vanholme</name>
<affiliation wicri:level="4"><nlm:affiliation>Ghent University, Department of Plant Biotechnology and Bioinformatics, 9052 Ghent, Belgium.</nlm:affiliation>
<country xml:lang="fr">Belgique</country>
<wicri:regionArea>Ghent University, Department of Plant Biotechnology and Bioinformatics, 9052 Ghent</wicri:regionArea>
<orgName type="university">Université de Gand</orgName>
<placeName><settlement type="city">Gand</settlement>
<region>Région flamande</region>
<region type="district" nuts="2">Province de Flandre-Orientale</region>
</placeName>
</affiliation>
<affiliation wicri:level="1"><nlm:affiliation>VIB Center for Plant Systems Biology, 9052 Ghent, Belgium.</nlm:affiliation>
<country xml:lang="fr">Belgique</country>
<wicri:regionArea>VIB Center for Plant Systems Biology, 9052 Ghent</wicri:regionArea>
<wicri:noRegion>9052 Ghent</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Goeminne, Geert" sort="Goeminne, Geert" uniqKey="Goeminne G" first="Geert" last="Goeminne">Geert Goeminne</name>
<affiliation wicri:level="4"><nlm:affiliation>Ghent University, Department of Plant Biotechnology and Bioinformatics, 9052 Ghent, Belgium.</nlm:affiliation>
<country xml:lang="fr">Belgique</country>
<wicri:regionArea>Ghent University, Department of Plant Biotechnology and Bioinformatics, 9052 Ghent</wicri:regionArea>
<orgName type="university">Université de Gand</orgName>
<placeName><settlement type="city">Gand</settlement>
<region>Région flamande</region>
<region type="district" nuts="2">Province de Flandre-Orientale</region>
</placeName>
</affiliation>
<affiliation wicri:level="1"><nlm:affiliation>VIB Center for Plant Systems Biology, 9052 Ghent, Belgium.</nlm:affiliation>
<country xml:lang="fr">Belgique</country>
<wicri:regionArea>VIB Center for Plant Systems Biology, 9052 Ghent</wicri:regionArea>
<wicri:noRegion>9052 Ghent</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Van Acker, Rebecca" sort="Van Acker, Rebecca" uniqKey="Van Acker R" first="Rebecca" last="Van Acker">Rebecca Van Acker</name>
<affiliation wicri:level="4"><nlm:affiliation>Ghent University, Department of Plant Biotechnology and Bioinformatics, 9052 Ghent, Belgium.</nlm:affiliation>
<country xml:lang="fr">Belgique</country>
<wicri:regionArea>Ghent University, Department of Plant Biotechnology and Bioinformatics, 9052 Ghent</wicri:regionArea>
<orgName type="university">Université de Gand</orgName>
<placeName><settlement type="city">Gand</settlement>
<region>Région flamande</region>
<region type="district" nuts="2">Province de Flandre-Orientale</region>
</placeName>
</affiliation>
<affiliation wicri:level="1"><nlm:affiliation>VIB Center for Plant Systems Biology, 9052 Ghent, Belgium.</nlm:affiliation>
<country xml:lang="fr">Belgique</country>
<wicri:regionArea>VIB Center for Plant Systems Biology, 9052 Ghent</wicri:regionArea>
<wicri:noRegion>9052 Ghent</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Fonseca, Fernando Campos De Assis" sort="Fonseca, Fernando Campos De Assis" uniqKey="Fonseca F" first="Fernando Campos De Assis" last="Fonseca">Fernando Campos De Assis Fonseca</name>
<affiliation wicri:level="4"><nlm:affiliation>Ghent University, Department of Plant Biotechnology and Bioinformatics, 9052 Ghent, Belgium.</nlm:affiliation>
<country xml:lang="fr">Belgique</country>
<wicri:regionArea>Ghent University, Department of Plant Biotechnology and Bioinformatics, 9052 Ghent</wicri:regionArea>
<orgName type="university">Université de Gand</orgName>
<placeName><settlement type="city">Gand</settlement>
<region>Région flamande</region>
<region type="district" nuts="2">Province de Flandre-Orientale</region>
</placeName>
</affiliation>
<affiliation wicri:level="1"><nlm:affiliation>VIB Center for Plant Systems Biology, 9052 Ghent, Belgium.</nlm:affiliation>
<country xml:lang="fr">Belgique</country>
<wicri:regionArea>VIB Center for Plant Systems Biology, 9052 Ghent</wicri:regionArea>
<wicri:noRegion>9052 Ghent</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Pallidis, Andreas" sort="Pallidis, Andreas" uniqKey="Pallidis A" first="Andreas" last="Pallidis">Andreas Pallidis</name>
<affiliation wicri:level="4"><nlm:affiliation>Ghent University, Department of Plant Biotechnology and Bioinformatics, 9052 Ghent, Belgium.</nlm:affiliation>
<country xml:lang="fr">Belgique</country>
<wicri:regionArea>Ghent University, Department of Plant Biotechnology and Bioinformatics, 9052 Ghent</wicri:regionArea>
<orgName type="university">Université de Gand</orgName>
<placeName><settlement type="city">Gand</settlement>
<region>Région flamande</region>
<region type="district" nuts="2">Province de Flandre-Orientale</region>
</placeName>
</affiliation>
<affiliation wicri:level="1"><nlm:affiliation>VIB Center for Plant Systems Biology, 9052 Ghent, Belgium.</nlm:affiliation>
<country xml:lang="fr">Belgique</country>
<wicri:regionArea>VIB Center for Plant Systems Biology, 9052 Ghent</wicri:regionArea>
<wicri:noRegion>9052 Ghent</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Voorend, Wannes" sort="Voorend, Wannes" uniqKey="Voorend W" first="Wannes" last="Voorend">Wannes Voorend</name>
<affiliation wicri:level="4"><nlm:affiliation>Ghent University, Department of Plant Biotechnology and Bioinformatics, 9052 Ghent, Belgium.</nlm:affiliation>
<country xml:lang="fr">Belgique</country>
<wicri:regionArea>Ghent University, Department of Plant Biotechnology and Bioinformatics, 9052 Ghent</wicri:regionArea>
<orgName type="university">Université de Gand</orgName>
<placeName><settlement type="city">Gand</settlement>
<region>Région flamande</region>
<region type="district" nuts="2">Province de Flandre-Orientale</region>
</placeName>
</affiliation>
<affiliation wicri:level="1"><nlm:affiliation>VIB Center for Plant Systems Biology, 9052 Ghent, Belgium.</nlm:affiliation>
<country xml:lang="fr">Belgique</country>
<wicri:regionArea>VIB Center for Plant Systems Biology, 9052 Ghent</wicri:regionArea>
<wicri:noRegion>9052 Ghent</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Junior, Jose Nicomedes" sort="Junior, Jose Nicomedes" uniqKey="Junior J" first="José Nicomedes" last="Junior">José Nicomedes Junior</name>
<affiliation wicri:level="4"><nlm:affiliation>Ghent University, Department of Plant Biotechnology and Bioinformatics, 9052 Ghent, Belgium.</nlm:affiliation>
<country xml:lang="fr">Belgique</country>
<wicri:regionArea>Ghent University, Department of Plant Biotechnology and Bioinformatics, 9052 Ghent</wicri:regionArea>
<orgName type="university">Université de Gand</orgName>
<placeName><settlement type="city">Gand</settlement>
<region>Région flamande</region>
<region type="district" nuts="2">Province de Flandre-Orientale</region>
</placeName>
</affiliation>
<affiliation wicri:level="1"><nlm:affiliation>VIB Center for Plant Systems Biology, 9052 Ghent, Belgium.</nlm:affiliation>
<country xml:lang="fr">Belgique</country>
<wicri:regionArea>VIB Center for Plant Systems Biology, 9052 Ghent</wicri:regionArea>
<wicri:noRegion>9052 Ghent</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:affiliation>Petróleo Brasileiro S.A., Centro de Pesquisas Leopoldo Américo Miguez de Mello, Rio de Janeiro, 21941-598, Brazil.</nlm:affiliation>
<country xml:lang="fr">Brésil</country>
<wicri:regionArea>Petróleo Brasileiro S.A., Centro de Pesquisas Leopoldo Américo Miguez de Mello, Rio de Janeiro, 21941-598</wicri:regionArea>
<wicri:noRegion>21941-598</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Padmakshan, Dharshana" sort="Padmakshan, Dharshana" uniqKey="Padmakshan D" first="Dharshana" last="Padmakshan">Dharshana Padmakshan</name>
<affiliation wicri:level="2"><nlm:affiliation>Department of Energy Great Lakes Bioenergy Research Center, Wisconsin Energy Institute, University of Wisconsin, Madison, Wisconsin 53726.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Wisconsin</region>
</placeName>
<wicri:cityArea>Department of Energy Great Lakes Bioenergy Research Center, Wisconsin Energy Institute, University of Wisconsin, Madison</wicri:cityArea>
</affiliation>
<affiliation wicri:level="2"><nlm:affiliation>Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Wisconsin</region>
</placeName>
<wicri:cityArea>Department of Biochemistry, University of Wisconsin, Madison</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Van Doorsselaere, Jan" sort="Van Doorsselaere, Jan" uniqKey="Van Doorsselaere J" first="Jan" last="Van Doorsselaere">Jan Van Doorsselaere</name>
<affiliation wicri:level="1"><nlm:affiliation>VIVES, 8800 Roeselare, Belgium.</nlm:affiliation>
<country xml:lang="fr">Belgique</country>
<wicri:regionArea>VIVES, 8800 Roeselare</wicri:regionArea>
<wicri:noRegion>8800 Roeselare</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Ralph, John" sort="Ralph, John" uniqKey="Ralph J" first="John" last="Ralph">John Ralph</name>
<affiliation wicri:level="2"><nlm:affiliation>Department of Energy Great Lakes Bioenergy Research Center, Wisconsin Energy Institute, University of Wisconsin, Madison, Wisconsin 53726.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Wisconsin</region>
</placeName>
<wicri:cityArea>Department of Energy Great Lakes Bioenergy Research Center, Wisconsin Energy Institute, University of Wisconsin, Madison</wicri:cityArea>
</affiliation>
<affiliation wicri:level="2"><nlm:affiliation>Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Wisconsin</region>
</placeName>
<wicri:cityArea>Department of Biochemistry, University of Wisconsin, Madison</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Boerjan, Wout" sort="Boerjan, Wout" uniqKey="Boerjan W" first="Wout" last="Boerjan">Wout Boerjan</name>
<affiliation wicri:level="4"><nlm:affiliation>Ghent University, Department of Plant Biotechnology and Bioinformatics, 9052 Ghent, Belgium woboe@psb.vib-ugent.be.</nlm:affiliation>
<country wicri:rule="url">Belgique</country>
<wicri:regionArea>Ghent University, Department of Plant Biotechnology and Bioinformatics, 9052 Ghent</wicri:regionArea>
<orgName type="university">Université de Gand</orgName>
<placeName><settlement type="city">Gand</settlement>
<region>Région flamande</region>
<region type="district" nuts="2">Province de Flandre-Orientale</region>
</placeName>
</affiliation>
<affiliation wicri:level="1"><nlm:affiliation>VIB Center for Plant Systems Biology, 9052 Ghent, Belgium.</nlm:affiliation>
<country xml:lang="fr">Belgique</country>
<wicri:regionArea>VIB Center for Plant Systems Biology, 9052 Ghent</wicri:regionArea>
<wicri:noRegion>9052 Ghent</wicri:noRegion>
</affiliation>
</author>
</analytic>
<series><title level="j">Plant physiology</title>
<idno type="eISSN">1532-2548</idno>
<imprint><date when="2017" type="published">2017</date>
</imprint>
</series>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biomass (MeSH)</term>
<term>Carbohydrate Metabolism (genetics)</term>
<term>Cellulose (metabolism)</term>
<term>Down-Regulation (genetics)</term>
<term>Esterases (metabolism)</term>
<term>Gene Expression Regulation, Plant (MeSH)</term>
<term>Gene Silencing (MeSH)</term>
<term>Genes, Plant (MeSH)</term>
<term>Lignin (metabolism)</term>
<term>Magnetic Resonance Spectroscopy (MeSH)</term>
<term>Metabolic Networks and Pathways (MeSH)</term>
<term>Phenols (metabolism)</term>
<term>Plant Development (genetics)</term>
<term>Plant Proteins (metabolism)</term>
<term>Plants, Genetically Modified (MeSH)</term>
<term>Populus (enzymology)</term>
<term>Populus (genetics)</term>
<term>Populus (growth & development)</term>
<term>Shikimic Acid (metabolism)</term>
<term>Xylem (metabolism)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Acide shikimique (métabolisme)</term>
<term>Biomasse (MeSH)</term>
<term>Cellulose (métabolisme)</term>
<term>Développement des plantes (génétique)</term>
<term>Esterases (métabolisme)</term>
<term>Extinction de l'expression des gènes (MeSH)</term>
<term>Gènes de plante (MeSH)</term>
<term>Lignine (métabolisme)</term>
<term>Métabolisme glucidique (génétique)</term>
<term>Phénols (métabolisme)</term>
<term>Populus (croissance et développement)</term>
<term>Populus (enzymologie)</term>
<term>Populus (génétique)</term>
<term>Protéines végétales (métabolisme)</term>
<term>Régulation de l'expression des gènes végétaux (MeSH)</term>
<term>Régulation négative (génétique)</term>
<term>Spectroscopie par résonance magnétique (MeSH)</term>
<term>Voies et réseaux métaboliques (MeSH)</term>
<term>Végétaux génétiquement modifiés (MeSH)</term>
<term>Xylème (métabolisme)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Cellulose</term>
<term>Esterases</term>
<term>Lignin</term>
<term>Phenols</term>
<term>Plant Proteins</term>
<term>Shikimic Acid</term>
</keywords>
<keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Populus</term>
</keywords>
<keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Populus</term>
</keywords>
<keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Populus</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Carbohydrate Metabolism</term>
<term>Down-Regulation</term>
<term>Plant Development</term>
<term>Populus</term>
</keywords>
<keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Populus</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Développement des plantes</term>
<term>Métabolisme glucidique</term>
<term>Populus</term>
<term>Régulation négative</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Xylem</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Acide shikimique</term>
<term>Cellulose</term>
<term>Esterases</term>
<term>Lignine</term>
<term>Phénols</term>
<term>Protéines végétales</term>
<term>Xylème</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Biomass</term>
<term>Gene Expression Regulation, Plant</term>
<term>Gene Silencing</term>
<term>Genes, Plant</term>
<term>Magnetic Resonance Spectroscopy</term>
<term>Metabolic Networks and Pathways</term>
<term>Plants, Genetically Modified</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Biomasse</term>
<term>Extinction de l'expression des gènes</term>
<term>Gènes de plante</term>
<term>Régulation de l'expression des gènes végétaux</term>
<term>Spectroscopie par résonance magnétique</term>
<term>Voies et réseaux métaboliques</term>
<term>Végétaux génétiquement modifiés</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Caffeoyl shikimate esterase (CSE) was recently shown to play an essential role in lignin biosynthesis in Arabidopsis (<i>Arabidopsis thaliana</i>
) and later in <i>Medicago truncatula</i>
 However, the general function of this enzyme was recently questioned by the apparent lack of CSE activity in lignifying tissues of different plant species. Here, we show that down-regulation of <i>CSE</i>
 in hybrid poplar (<i>Populus tremula</i>
 × <i>Populus alba</i>
) resulted in up to 25% reduced lignin deposition, increased levels of <i>p</i>
-hydroxyphenyl units in the lignin polymer, and a relatively higher cellulose content. The transgenic trees were morphologically indistinguishable from the wild type. Ultra-high-performance liquid chromatography-mass spectrometry-based phenolic profiling revealed a reduced abundance of several oligolignols containing guaiacyl and syringyl units and their corresponding hydroxycinnamaldehyde units, in agreement with the reduced flux toward coniferyl and sinapyl alcohol. These trees accumulated the CSE substrate caffeoyl shikimate along with other compounds belonging to the metabolic classes of benzenoids and hydroxycinnamates. Furthermore, the reduced lignin amount combined with the relative increase in cellulose content in the <i>CSE</i>
 down-regulated lines resulted in up to 62% more glucose released per plant upon limited saccharification when no pretreatment was applied and by up to 86% and 91% when acid and alkaline pretreatments were used. Our results show that CSE is not only important for the lignification process in poplar but is also a promising target for the development of improved lignocellulosic biomass crops for sugar platform biorefineries.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">28878037</PMID>
<DateCompleted><Year>2018</Year>
<Month>07</Month>
<Day>03</Day>
</DateCompleted>
<DateRevised><Year>2018</Year>
<Month>11</Month>
<Day>13</Day>
</DateRevised>
<Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1532-2548</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>175</Volume>
<Issue>3</Issue>
<PubDate><Year>2017</Year>
<Month>Nov</Month>
</PubDate>
</JournalIssue>
<Title>Plant physiology</Title>
<ISOAbbreviation>Plant Physiol</ISOAbbreviation>
</Journal>
<ArticleTitle>Silencing <i>CAFFEOYL SHIKIMATE ESTERASE</i>
 Affects Lignification and Improves Saccharification in Poplar.</ArticleTitle>
<Pagination><MedlinePgn>1040-1057</MedlinePgn>
</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.1104/pp.17.00920</ELocationID>
<Abstract><AbstractText>Caffeoyl shikimate esterase (CSE) was recently shown to play an essential role in lignin biosynthesis in Arabidopsis (<i>Arabidopsis thaliana</i>
) and later in <i>Medicago truncatula</i>
 However, the general function of this enzyme was recently questioned by the apparent lack of CSE activity in lignifying tissues of different plant species. Here, we show that down-regulation of <i>CSE</i>
 in hybrid poplar (<i>Populus tremula</i>
 × <i>Populus alba</i>
) resulted in up to 25% reduced lignin deposition, increased levels of <i>p</i>
-hydroxyphenyl units in the lignin polymer, and a relatively higher cellulose content. The transgenic trees were morphologically indistinguishable from the wild type. Ultra-high-performance liquid chromatography-mass spectrometry-based phenolic profiling revealed a reduced abundance of several oligolignols containing guaiacyl and syringyl units and their corresponding hydroxycinnamaldehyde units, in agreement with the reduced flux toward coniferyl and sinapyl alcohol. These trees accumulated the CSE substrate caffeoyl shikimate along with other compounds belonging to the metabolic classes of benzenoids and hydroxycinnamates. Furthermore, the reduced lignin amount combined with the relative increase in cellulose content in the <i>CSE</i>
 down-regulated lines resulted in up to 62% more glucose released per plant upon limited saccharification when no pretreatment was applied and by up to 86% and 91% when acid and alkaline pretreatments were used. Our results show that CSE is not only important for the lignification process in poplar but is also a promising target for the development of improved lignocellulosic biomass crops for sugar platform biorefineries.</AbstractText>
<CopyrightInformation>© 2017 American Society of Plant Biologists. All Rights Reserved.</CopyrightInformation>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Saleme</LastName>
<ForeName>Marina de Lyra Soriano</ForeName>
<Initials>MLS</Initials>
<Identifier Source="ORCID">http://orcid.org/0000-0001-6526-0055</Identifier>
<AffiliationInfo><Affiliation>Ghent University, Department of Plant Biotechnology and Bioinformatics, 9052 Ghent, Belgium.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>VIB Center for Plant Systems Biology, 9052 Ghent, Belgium.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Cesarino</LastName>
<ForeName>Igor</ForeName>
<Initials>I</Initials>
<Identifier Source="ORCID">http://orcid.org/0000-0002-6789-2432</Identifier>
<AffiliationInfo><Affiliation>Ghent University, Department of Plant Biotechnology and Bioinformatics, 9052 Ghent, Belgium.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>VIB Center for Plant Systems Biology, 9052 Ghent, Belgium.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Department of Botany, Institute of Biosciences, University of São Paulo, 05508-090 Butanta, Sao Paulo, Brazil.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Vargas</LastName>
<ForeName>Lívia</ForeName>
<Initials>L</Initials>
<Identifier Source="ORCID">http://orcid.org/0000-0001-6334-8782</Identifier>
<AffiliationInfo><Affiliation>Ghent University, Department of Plant Biotechnology and Bioinformatics, 9052 Ghent, Belgium.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>VIB Center for Plant Systems Biology, 9052 Ghent, Belgium.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Kim</LastName>
<ForeName>Hoon</ForeName>
<Initials>H</Initials>
<Identifier Source="ORCID">http://orcid.org/0000-0001-7425-7464</Identifier>
<AffiliationInfo><Affiliation>Department of Energy Great Lakes Bioenergy Research Center, Wisconsin Energy Institute, University of Wisconsin, Madison, Wisconsin 53726.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Vanholme</LastName>
<ForeName>Ruben</ForeName>
<Initials>R</Initials>
<Identifier Source="ORCID">http://orcid.org/0000-0001-5848-3138</Identifier>
<AffiliationInfo><Affiliation>Ghent University, Department of Plant Biotechnology and Bioinformatics, 9052 Ghent, Belgium.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>VIB Center for Plant Systems Biology, 9052 Ghent, Belgium.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Goeminne</LastName>
<ForeName>Geert</ForeName>
<Initials>G</Initials>
<Identifier Source="ORCID">http://orcid.org/0000-0002-0337-2999</Identifier>
<AffiliationInfo><Affiliation>Ghent University, Department of Plant Biotechnology and Bioinformatics, 9052 Ghent, Belgium.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>VIB Center for Plant Systems Biology, 9052 Ghent, Belgium.</Affiliation>
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<name sortKey="Fonseca, Fernando Campos De Assis" sort="Fonseca, Fernando Campos De Assis" uniqKey="Fonseca F" first="Fernando Campos De Assis" last="Fonseca">Fernando Campos De Assis Fonseca</name>
<name sortKey="Goeminne, Geert" sort="Goeminne, Geert" uniqKey="Goeminne G" first="Geert" last="Goeminne">Geert Goeminne</name>
<name sortKey="Goeminne, Geert" sort="Goeminne, Geert" uniqKey="Goeminne G" first="Geert" last="Goeminne">Geert Goeminne</name>
<name sortKey="Junior, Jose Nicomedes" sort="Junior, Jose Nicomedes" uniqKey="Junior J" first="José Nicomedes" last="Junior">José Nicomedes Junior</name>
<name sortKey="Junior, Jose Nicomedes" sort="Junior, Jose Nicomedes" uniqKey="Junior J" first="José Nicomedes" last="Junior">José Nicomedes Junior</name>
<name sortKey="Pallidis, Andreas" sort="Pallidis, Andreas" uniqKey="Pallidis A" first="Andreas" last="Pallidis">Andreas Pallidis</name>
<name sortKey="Pallidis, Andreas" sort="Pallidis, Andreas" uniqKey="Pallidis A" first="Andreas" last="Pallidis">Andreas Pallidis</name>
<name sortKey="Saleme, Marina De Lyra Soriano" sort="Saleme, Marina De Lyra Soriano" uniqKey="Saleme M" first="Marina De Lyra Soriano" last="Saleme">Marina De Lyra Soriano Saleme</name>
<name sortKey="Van Acker, Rebecca" sort="Van Acker, Rebecca" uniqKey="Van Acker R" first="Rebecca" last="Van Acker">Rebecca Van Acker</name>
<name sortKey="Van Acker, Rebecca" sort="Van Acker, Rebecca" uniqKey="Van Acker R" first="Rebecca" last="Van Acker">Rebecca Van Acker</name>
<name sortKey="Van Doorsselaere, Jan" sort="Van Doorsselaere, Jan" uniqKey="Van Doorsselaere J" first="Jan" last="Van Doorsselaere">Jan Van Doorsselaere</name>
<name sortKey="Vanholme, Ruben" sort="Vanholme, Ruben" uniqKey="Vanholme R" first="Ruben" last="Vanholme">Ruben Vanholme</name>
<name sortKey="Vanholme, Ruben" sort="Vanholme, Ruben" uniqKey="Vanholme R" first="Ruben" last="Vanholme">Ruben Vanholme</name>
<name sortKey="Vargas, Livia" sort="Vargas, Livia" uniqKey="Vargas L" first="Lívia" last="Vargas">Lívia Vargas</name>
<name sortKey="Vargas, Livia" sort="Vargas, Livia" uniqKey="Vargas L" first="Lívia" last="Vargas">Lívia Vargas</name>
<name sortKey="Voorend, Wannes" sort="Voorend, Wannes" uniqKey="Voorend W" first="Wannes" last="Voorend">Wannes Voorend</name>
<name sortKey="Voorend, Wannes" sort="Voorend, Wannes" uniqKey="Voorend W" first="Wannes" last="Voorend">Wannes Voorend</name>
</country>
<country name="Brésil"><region name="État de São Paulo"><name sortKey="Cesarino, Igor" sort="Cesarino, Igor" uniqKey="Cesarino I" first="Igor" last="Cesarino">Igor Cesarino</name>
</region>
<name sortKey="Junior, Jose Nicomedes" sort="Junior, Jose Nicomedes" uniqKey="Junior J" first="José Nicomedes" last="Junior">José Nicomedes Junior</name>
</country>
<country name="États-Unis"><region name="Wisconsin"><name sortKey="Kim, Hoon" sort="Kim, Hoon" uniqKey="Kim H" first="Hoon" last="Kim">Hoon Kim</name>
</region>
<name sortKey="Kim, Hoon" sort="Kim, Hoon" uniqKey="Kim H" first="Hoon" last="Kim">Hoon Kim</name>
<name sortKey="Padmakshan, Dharshana" sort="Padmakshan, Dharshana" uniqKey="Padmakshan D" first="Dharshana" last="Padmakshan">Dharshana Padmakshan</name>
<name sortKey="Padmakshan, Dharshana" sort="Padmakshan, Dharshana" uniqKey="Padmakshan D" first="Dharshana" last="Padmakshan">Dharshana Padmakshan</name>
<name sortKey="Ralph, John" sort="Ralph, John" uniqKey="Ralph J" first="John" last="Ralph">John Ralph</name>
<name sortKey="Ralph, John" sort="Ralph, John" uniqKey="Ralph J" first="John" last="Ralph">John Ralph</name>
</country>
</tree>
</affiliations>
</record>

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{{Explor lien
   |wiki=    Bois
   |area=    PoplarV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     pubmed:28878037
   |texte=   Silencing CAFFEOYL SHIKIMATE ESTERASE Affects Lignification and Improves Saccharification in Poplar.
}}

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Serveur d'exploration sur le peuplier

Silencing CAFFEOYL SHIKIMATE ESTERASE Affects Lignification and Improves Saccharification in Poplar.

Silencing CAFFEOYL SHIKIMATE ESTERASE Affects Lignification and Improves Saccharification in Poplar.

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